Small molecule inhibition of Ewing sarcoma cell growth via targeting the long non coding RNA HULC
Neri Mercatelli, Diana Fortini, Ramona Palombo, Maria Paola Paronetto
PII: S0304-3835(19)30523-3
DOI: https://doi.org/10.1016/j.canlet.2019.10.026 Reference: CAN 114533
To appear in: Cancer Letters
Received Date: 26 July 2019
Revised Date: 16 October 2019
Accepted Date: 17 October 2019
Please cite this article as: N. Mercatelli, D. Fortini, R. Palombo, M.P. Paronetto, Small molecule inhibition of Ewing sarcoma cell growth via targeting the long non coding RNA HULC, Cancer Letters, https://doi.org/10.1016/j.canlet.2019.10.026.
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Abstract
Ewing sarcomas (ES) are aggressive pediatric cancers of bone and soft tissues characterized by in frame chromosomal translocations giving rise to chimeric transcription factors, such as EWS-FLI1. An emerging strategy to block EWS-FLI1 activity is represented by the small molecule YK-4-279, which binds to EWS-FLI1 and alters its transcriptional activity. The specific effectors of the anti-oncogenic activity of YK-4-279 are still largely unknown. Herein, by performing a high-throughput screening we identify the lncRNA HULC (Highly Upregulated in Liver Cancer) as a prominent target of YK-4-279 activity in ES cells. High levels of HULC correlate with ES aggressiveness, whereas HULC depletion reduces ES cell growth. Mechanistically, we find that HULC promotes the expression of TWIST1 oncogene by sponging miR-186. Downregulation of HULC upon treatment with YK-4-279 reduces the expression of TWIST1 by unleashing miR-186 and favoring its binding to TWIST1 transcripts. Notably, high levels of miR-186 and low levels of TWIST1 correlate with better prognosis in ES patients.
Our results disclose a novel oncogenic regulatory circuit mediated by HULC lncRNA that is disrupted by the small molecule YK-4-279, with promising therapeutic implications for ES treatment.
1Laboratory of Molecular and Cellular Neurobiology, IRCCS Santa Lucia Foundation, Rome 00143, Italy;
2Department of Movement, Human and Health Sciences, University of Rome “Foro Italico”, Piazza Lauro de Bosis 6, 00135, Rome, Italy;
Corresponding authors:
Maria Paola Paronetto: [email protected]
Neri Mercatelli: [email protected]
Running Title: HULC mediates YK-4-279 response Keywords: Ewing sarcoma, noncoding RNA, HULC, TWIST1
Abbreviations:
ES: Ewing sarcoma, EWS: Ewing sarcoma protein, FLI1: Friend Leukemia virus integration site 1, NR0B1: Nuclear Receptor Subfamily 0 Group B Member 1, GLI1: GLI Family Zinc Finger 1, LOX: Lysyl Oxidase, FOX1: Forkhead Box O1, FOXM1: Forkhead Box M1, RHA: RNA helicase A, Bcl-2: B-cell lymphoma 2, lncRNAs: long noncoding RNAs, ceRNAs: competing endogenous RNAs, miRNAs: microRNAs, HULC: Highly up-regulated in liver
cancer, ASO: antisense oligonucleotide, AFAP-1AS1: Actin Filament Associated Protein 1 Antisense 1, SDS: sodium dodecyl sulfate, PI: propidium iodide, CCND1: CyclinD1 ID2: Inhibitor of DNA Binding 2, c-MYC: MYC Proto Oncogene, TGFBR1: Transforming Growth Factor Beta Receptor 1, CCAT2: Colon Cancer Associated Transcript 2, SUMO1P3: SUMO1 Pseudogene 3, HIF1A-AS2: HIF1A Antisense RNA 2, MIR17-HG: miR-17 Host Gene, NRON: Non-Coding Repressor Of NFAT, MALAT: Metastasis Associated Lung Adenocarcinoma Transcript 1, NEAT1: Nuclear Paraspeckle Assembly Transcript 1, CCAT1: Colon Cancer Associated Transcript 1, H19: H19 Imprinted Maternally Expressed Transcript, HMGA2: High Mobility Group AT-Hook 2
Abstract
Ewing sarcomas (ES) are aggressive pediatric cancers of bone and soft tissues characterized by in frame chromosomal translocations giving rise to chimeric transcription factors, such as EWS-FLI1. An emerging strategy to block EWS-FLI1 activity is represented by the small molecule YK-4-279, which binds to EWS-FLI1 and alters its transcriptional activity. The specific effectors of the anti-oncogenic activity of YK-4-279 are still largely unknown. Herein, by performing a high-throughput screening we identify the lncRNA HULC (Highly Upregulated in Liver Cancer) as a prominent target of YK-4-279 activity in ES cells. High levels of HULC correlate with ES aggressiveness, whereas HULC depletion reduces ES cell growth. Mechanistically, we find that HULC promotes the expression of TWIST1 oncogene by sponging miR-186. Downregulation of HULC upon treatment with YK-4-279 reduces the expression of TWIST1 by unleashing miR-186 and favoring its binding to TWIST1 transcripts. Notably, high levels of miR-186 and low levels of TWIST1 correlate with better prognosis in ES patients.
Our results disclose a novel oncogenic regulatory circuit mediated by HULC lncRNA that is disrupted by the small molecule YK-4-279, with promising therapeutic implications for ES treatment.
⦁ Introduction
Ewing sarcoma (ES) is a rare and highly aggressive cancer of bone and soft tissues predominantly affecting children and adolescents between the ages of 10 and 25 [1]. ES tumors are characterized by recurrent chromosomal translocations yielding in frame fusion proteins which consist of the amino terminus of the Ewing Sarcoma Protein (EWS) joined to the carboxyl terminus of different transcription factors belonging to the ETS-domain family [2]. The most common translocation is originated by the fusion of EWSR1 gene, located on chromosome 22, to FLI1 (Friend Leukemia virus integration site 1) gene, on chromosome 11 [3]. This translocation gives rise to the chimeric oncogene EWS-FLI1 which acts as an aberrant transcription factor [4], promoting ES pathogenesis and regulating the expression of genes involved in cancer transformation, such as NR0B1, GLI1, LOX, FOX1, FOXOM1 [5, 6]. The oncogenic program of EWS-FLI1 is enhanced by its interaction with several molecular partners. Among them, the RNA helicase A (RHA), also called DHX9, plays a pivotal role in cancer progression and drug resistance [7, 8]. In particular, DHX9 enhances EWS-FLI1- mediated transcription and promotes anchorage-independent growth of ES cells [9, 10]. Conversely, DHX9 down-regulation sensitizes ES cells to genotoxic stress [11]. In line with this, molecular strategies targeting the association between EWS-FLI1 and DHX9 are emerging for their potential implication in ES pathology [12]. Particularly, the chemical compound YK-4-279 is a recently synthetized molecule displaying a powerful inhibitory effect on ES tumorigenicity by binding to EWS-FLI1 and disrupting its interaction with DHX9 [10, 13]. YK-4-279 administration increases the stability of cell cycle regulator Cyclin B1 and the expression of the proapoptotic isoforms of BCL2 and MCL1, thus reducing ES cellular growth both in vitro and in vivo and promoting apoptosis [10, 14]. However, the molecular mechanism
underlying the antitumorigenic activity of YK-4-279 is still far to be fully elucidated and novel molecular players could be involved in this regulation.
In the last years, long noncoding RNAs (lncRNAs) are emerging as a novel class of regulatory non coding RNAs [15]. By definition, lncRNAs are longer than 200 nucleotides and regulate gene expression at both transcriptional and post-transcriptional level [16]. Their mechanism of action depends on the intracellular localization and on the molecular partners involved [16]. In the nucleus, lncRNAs can interface with various chromatin-modifying enzymes, specific transcription factors, and with the RNA Polymerase II, thus globally affecting gene expression [16]. In addition, lncRNAs can act in cis by modulating the transcription of closely located genes [17]. Moreover, they have been implicated in splicing, export, turnover and processing of mRNA transcripts [16, 17]. In the last years, growing evidence documented the ability of many cytoplasmic lncRNAs in sequestering specific microRNAs (miRNAs), thus functioning as competing endogenous RNAs (ceRNAs) [18, 19]. Through complementary base-pairing, lncRNAs can titrate the free amount of one or more specific miRNAs, thus affecting the rate of targeted mRNAs [16, 20]. In line with this, a variety of lncRNAs have been identified as critical modulators of oncogenes and/or tumor suppressors through a miRNA-mediated post transcriptional mechanism. The aberrant expression of specific lncRNAs has been linked to different processes of malignant transformation, such as proliferation, apoptosis, invasion and migration [17, 21, 22].
Searching for lncRNAs modulated by YK-4-279 treatment, we identified HULC (Highly up-regulated in liver cancer) lncRNA as a critical modulator of ES chemoresistance. HULC is downregulated in ES cells after YK-4-279 exposure and its expression correlates with the aggressiveness of ES cells. Remarkably, we found that HULC ablation reduces ES cells
proliferation and clonogenicity. Mechanistically, we found that HULC affects the expression levels of TWIST1 oncogene by sponging miR-186, which acts as a tumor suppressor in ES cells. The pharmacological downregulation of HULC by YK-4-279 reduces the intracellular levels of TWIST1 protein by favoring the rate of miR-186 bound to its transcript. As a result, the downregulation of TWIST1 sensitizes ES cells to YK-4-279 treatment.
Collectively, our results depict a novel molecular axis mediated by HULC lncRNA functionally involved in the antitumorigenic activity of YK-4-279, with promising therapeutic potential for ES anticancer strategies.
⦁ Materials and Methods
⦁ Cell cultures and drug treatment
ES cell line LAP-35 (RRID:CVCL_A096) was a generous gift from Drs. Katia Scotlandi and Cristina Manara [23]. ES cell line TC-71 (RRID:CVCL_2213), RD-ES (RRID:CVCL_2169) and SK-N-MC (RRID:CVCL_0530) were purchased from DSMZ. Absence of mycoplasma contamination was verified every two months by PCR analysis. All experiments were performed with mycoplasma-free cells. All human cell lines have been authenticated within the last three years. Cell lines were maintained in culture in Iscove’s modified Dulbecco’s medium (IMDM, GIBCO), supplemented with 10% fetal bovine serum, and penicillin and streptomycin (Gibco) and maintained at 37°C in humidified 5% CO2 atmosphere. For drug treatment, ES cells were treated for the indicated time with either DMSO or YK-4-279 (Cayman Chemical) at the indicated concentrations. To obtain YK-resistant TC-71 cells, a scaling-up treatment was performed. Briefly, cells were cultivated for 5 weeks with increasing concentration of YK-4-279, starting from 0.75 M to 2 M, and maintained at the same drug
concentration. A pulse treatment was performed at the end of the selection, and cellular viability was monitored after 48 hours by MTS assay. Doxorubicin (S1208), Etoposide (S1225) and Vincristine (S1241) were purchased from Selleckchem.
⦁ Transfections
Lipofectamine RNAiMax reagent (ThermoFisher) was used for Antisense oligonucleotides (ASO), siRNAs and miRNA mimics transfections. Briefly, 2×104 TC-71 cells were subjected to double pulse of reverse-transfection by using 4l of Lipofectamine RNAiMAX and cells were collected or re-plated for further experiments 24 hours after the last pulse of transfection. Plasmid transfections were performed on 80%-confluent cells by using Lipofectamine 2000, following manufacturer’s instructions (ThermoFisher). Stable TC-71 cells overexpressing TWIST1 were selected by adding 500g/ml of Geneticin (G418) directly into the culture media 48 hours after transfection. After selection, cells were used for the indicated experiments until the fifth passage, and then discarded.
Mirvana miRNA mimic hsa-miR-186-5p (MC11753), miRNA mimic hsa-miR-218-5p (MC10328), miRNA mimic hsa-miR-663a (MC11581) and miRNA mimic Negative Control #1 (4464060) were purchased from ThermoFisher.
TWIST1 siRNA was purchased by Sigma-Aldrich (EHU151571) and Antisense oligonucleotides (ASO) are listed in Supplementary Table S1.
⦁ Plasmid constructs
Nucleotide sequences of HULC and AFAP-1AS1 lncRNAs were PCR-amplified from genomic human DNA by using Phusion High-Fidelity DNA Polymerase (ThermoFisher) according to manufacturer’s instruction. The sequence of HULC (relative to the second exon
of its RNA sequence) containing the binding sites for miR-186 and miR-218 was cloned into XhoI and NotI sites of psiCHECK TM-2 vector (Promega) or in pcDNA TM3.1- (ThermoFisher) to generate psiHULC and pcHULC, respectively. AFAP1-AS1 sequence was cloned into BamHI site of pcDNA TM3.1+. The miRNA sensor plasmids (psi-miR-186 and psi-miR-218) were generated by annealing two complementary synthetic oligonucleotides corresponding to a portion (twice-repeated: 2X) of the reverse complement strand of premiR-186 or premiR-
218. The resulting double –strand DNAs containing cohesive ends for XhoI and NotI were cloned into XhoI and NotI sites of psiCHECK TM-2 vector.
The ligase reactions were performed by using T4 DNA Ligase (Promega) following the manufacturer’s instructions. pcTwist1 plasmid was a generous gift from Drs. Roberta Maestro and Sara Piccinin [24].
The sequence of primers and oligonucleotides used are listed in Supplementary Table S2. The sequence of PCR-derived fragments was confirmed by DNA sequence analysis.
⦁ Luciferase reporter assay
TC-71 cells were transfected in a 24-well format with 1g of psiHULC construct by using 2l of Lipofectamine 2000. After 24 hours, cells were detached, adjusted to a right concentration and reverse-transfected with miR-186, miR-218, miR-663a (5nM) or with a synthetic scramble-sequence mimic miRNA. 24 hours after plating, luciferase’s activity was measured by using Dual luciferase reporter assay system protocol (Promega), following manufacturer’s recommendations. In the competition assay, the miRNA sensor plasmids were co-transfected with increasing amount (0.5; 0.6; 0.8g) of pcHULC or pcDNA3.1. Values reported in the graphics refer to the ratio between renilla and firefly luciferase activities calculated as mean ± SD of at least three independent experiments.
⦁ SDS–PAGE and Western blot analyses
For protein extract preparation, cells were washed twice with ice-cold phosphate buffered saline (PBS), resuspended in RIPA lysis buffer [150 mM NaCl, 50 mM Tris-HCl pH 7.5, 2 mM EDTA, 0.1 % in sodium dodecyl sulfate (SDS), 0.5% sodium deoxycolate,1mM dithiothreitol, 0.5 mM Na-orthovanadate, 1%, 10 mM B-glycerolphosphate, 10 mM sodium fluoride , 1% NP-40 and Protease-Inhibitor Cocktail (Sigma-Aldrich)] and kept on ice for 10 min. Soluble protein extracts were separated by centrifugation at 12000 rpm for 10 min and diluted in Laemlli sample buffer. The obtained cell lysates were resolved on SDS– polyacrylamide gels (SDS-PAGE) and transferred on PVDF membrane Hybond TM-P (Amersham Bioscience). Membranes were saturated with 5% BSA at room temperature and incubated with the following primary antibodies at 4°C overnight: mouse GAPDH (SC-32233), mouse TWIST1 (SC-81417), rabbit FLI1 (ab15289) and rabbit HMGA2 (CST-5269). Secondary anti-mouse or anti-rabbit IgGs conjugated to horseradish peroxidase (Amersham) were incubated with the membranes for 1 hour at room temperature at a 1:10000 dilution. Immunostained bands were detected by a chemiluminescent method (Thermo Scientific).
⦁ RT−PCR analysis and Real-time quantitative PCR analyses
RNA was isolated and DNase digested using either Trizol (Invitrogen) or RNeasy kit (Qiagen). Total RNA (2g) was reverse transcribed by using SuperScript III Reverse Transcriptase (Invitrogen) following manufacturer’s instructions. RT reaction was used as template together with the different primers listed in Supplementary Table S3, and 35-40 cycles of amplifications were performed depending on the experiment. Primers were designed using Primer 3 Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi) and
Primer-Blast using the reference and the alternative RefSeq accession numbers. For Real- time quantitative PCR analysis, the primers were designed such that their annealing temperature was 60°C, generating single-amplification products in the range of 60- to 120- base-pairs (bp) long. PCR amplification was carried out with 1 µL of the 1:10 diluted reverse transcription sample with 10 µL of 2× SYBR Green Master Mix (Roche) and 4 pmol of specific gene primer pairs in a 20-µL total volume in 96-well microtiter plates. PCR reactions were run in triplicates on a LightCycler 480 system (Roche). Each experiment was performed at least in triplicate; data are represented as the mean ± standard deviation (SD). For all experiments, no-RT controls have been performed.
The Human Cancer PathwayFinder RT2 lncRNA PCR array (Qiagen) was used to profile the expression of 84 cancer-related lncRNAs. 2g of total RNA was reverse transcribed by RT2 First Strand Kit for 1 hour at 37°C. The synthetized cDNA was added to a PCR mix containing RT2 SYBR Green mastermix following manufacturer’s instructions (Qiagen). The mix was then dispensed into the RT2lncRNA PCR Array and PCR reactions were performed in triplicates on AB7900 HT instrument. Cycling conditions were: 10 min at 95°C and 40 cycles of 15 sec 95°C-1 min 60°C. Ct values for all wells were exported on a excel file and uploaded on to the data analysis web portal at: http://www.qiagen.com/geneglobe. Details of the data analyses were reported in the relevant figure legends.
⦁ Sub-cellular Fractionation
Sub-cellular fractionation was performed as described previously (25). Briefly, TC-71 cells were trypsinized and centrifuged for 5 minutes at 1000 rpm. Cell pellets were washed with PBS/1 mM EDTA, gently centrifuged and resuspended in ice-cold NP-40 lysis buffer (10 nM Tris-HCl pH 7.5, 0,15% NP-40, 150mM NaCl, protease inhibitor cocktail (Sigma-Aldrich),
1mM dithiothreitol, 0,5mM Na-orthovanadate) for 5 minutes. Lysates were layered on 2,5 volumes of chilled solution of 24% sucrose lysis buffer and centrifuged 10 minutes at 14000 rpm. The supernatant (cytoplasmic fraction) was collected and treated with proteinase K for 1 h at 37°C, then the RNA was extracted with phenol/chloroform and precipitated with ethanol. Pellets (nuclei) were washed with PBS/EDTA 1mM, gently centrifuged and resuspended in a chilled glycerol buffer (20mM Tris-HCl pH 7.9, 75 mM NaCl, 0,5mM EDTA, 0,85mM dithiothreitol, 0,125 mM PMSF and 50% Glycerol). An equal volume of cold nuclei lysis buffer (10 mM Hepes pH 7.6, 7,5 mM MgCl2, 0,2 mM EDTA, 0,3 M NaCl, 1 M UREA, 1% NP-40
protease inhibitor cocktail (Sigma-Aldrich), 1mM dithiothreitol, 0,5 mM Na-ortovanadate) was added, tubes were vortexed twice for 2 seconds and centrifuged for 2 minutes at 14 000 rpm. The supernatant (soluble nuclear fraction) was collected and treated with proteinase K for 1 h and 37°C, then extracted with phenol/chloroform and precipitated with ethanol. The chromatin pellet was gently rinsed with cold 1× PBS/1 mM EDTA and then dissolved in TRIzol (Invitrogen). All RNA fractions were resuspended in RNAse free water and quantified using a Nanodrop-1000 spectrophotometer (Nanodrop Technologies) and tested for DNA contamination by RT-PCR lacking reverse transcriptase. An equal amount of each fraction (1g) was reverse transcribed by using Reverse Transcriptase M-MLV (Promega) following manufacturer’s instructions and analyzed by RT-qPCR.
⦁ Clonogenic Assay
For clonogenic assay, single-cell suspensions were plated in 35mm plates at low density (1500 cells/plate). After 1 day, cells were treated with YK-4-279 as indicated in the figures. The medium was changed every 48-72 hours and YK-4-279 added at every change of medium. After 8 days, cells were fixed in methanol for 10 minutes and stained overnight
with 0.01% Crystal Violet solution (Sigma-Aldrich). Plates were then washed twice with water and dried. Pictures were acquired using digital camera to count the colonies. Results represent the mean ± S.D. of three experiments.
⦁ Cell growth and viability
MTS assay was performed to asses cellular viability after treatments. Briefly, 5X103 cells were plated in each well of a 96-culture plate and drugs were added in the culture media 24 hours later. The absorbance (OD 490nM) was measured after 24, 30 and 48 hours, by using Cell Titer Aqueous Assay (Promega) with MTS tetrazolium following manufacturer’s instructions. In cell survival experiments, the OD was measured at 48 hours and converted in cell number using a reference standard curve.
To generate the dose-response graph, the percentage of cell viability and the YK-4-279 logarithmic concentration were plotted. The IC50 was measured by using GraphPad Prism 8 through the non-linear regression method.
To monitor the effect of YK-4-279 on TC-71 cellular death, cells were harvested at 16, 24 and 48 hours after treatment and 1 g/ml of PI was added to each cell suspension. Flow cytometric analysis was performed using the FlowJo software, for the detection of percentage of PI stained cells.
Cellular growth of HULC depleted- TC-71 cells was measured by counting the total number of cells after 24, 48, 72 hours after HULC ASO or scr ASO transfections. Trypan blue dye was added to the cell suspension in a 1:1 volume ratio.
⦁ In-silico prediction for miRNA binding sites
The in-silico analysis for miRNA putative binding sites in HULC RNA sequence was performed by using microRNA.org tool (http://www.microrna.org/microrna/home.do) with the following setting; targeted mRNA search: HULC (NR_004855).
⦁ Kaplan Meier analysis
Event-free and overall survival analyses were performed on data from 64 ES patients
[25] by using R2: Genomics Analysis and Visualization Platform webcite (http://r2.amc.nl).
⦁ Results
⦁ HULC lncRNA is downregulated in Ewing sarcoma cells after YK-4-279 treatment
The oncogenic activity of EWS-FLI1 is strongly enhanced by its association with the DNA/RNA helicase DHX9 [9]. An emerging strategy in ES pathology is represented by the use of the small molecule YK-4-279, which binds EWS-FLI1 and prevents its interaction with DHX9 [10]. To determine the minimal time and dose required for the anti-oncogenic effects of YK-4-279, TC-71 ES cells were exposed to different concentrations of the drug and cellular viability was monitored by MTS assay. Treatment with YK-4-279 equal or higher than 2M dramatically decreased cell viability at 30 and 48 hours (Supplementary Fig. S1A). Propidium iodide (PI) staining was performed to directly evaluate the impact of YK-4-279 on cell death: the number of PI positive cells was strongly increased by treatment with 2, 3 or 10 M YK-4- 279 in comparison to DMSO controls at all the analyzed time points. Interestingly, robust induction of cell death was observed already at 24 hours, with a peak at 48 hours (2M ≈ 68%; 3M ≈ 75%; 10M ≈ 83%) (Supplementary Fig. S1B). To monitor the effect of YK-4-279 administration on EWS-FLI1 transcriptional activity, the expression of known EWS-FLI1/DHX9
targets was evaluated both at 16 and 24 hours of treatment. RT-qPCR analysis revealed that the mRNA levels encoded by several EWS-FLI1 target genes, such as CCND1, NR0B1, ID2 and c-MYC, were significantly reduced in TC-71 cells treated with 2M YK-4-279 starting from 16 hours of treatment, whereas no significant changes were observed for TGFBR1 negative control gene (Supplementary Fig. S1C). Taken together, these results show that 16 hour treatment with 2M YK-4-279 is sufficient to downregulate the expression of specific oncogenic mRNAs in ES cells and indicate that the effects of this small molecule on cell survival are temporally preceded by gene expression changes related to the dissociation of EWS-FLI1/DHX9 complex.
To understand the impact of YK-4-279 treatment on noncoding transcripts, we screened an RT-qPCR-based array containing probes for 84 cancer-related lncRNAs. The hierarchical clustering and Volcano plot analysis indicated that 26 lncRNAs were significantly modulated by treatment of TC71 cells with YK-4-279 (Fig. 1A,B), with 13 lncRNAs showing a change in expression higher than 1.5-fold (Fig.1C and Supplementary Fig.S1D). In particular, 6 of them were upregulated while 7 were downregulated (Fig.1C). Independent analysis by qPCR confirmed the effect of YK-4-279 on 9 of them (AFAP1-AS1, HULC, CCAT2, SUMO1P3, HIF1A-AS2, MIR17-HG, NRON, MALAT1, NEAT1), whereas expression changes of CCAT1 were not validated. The expression of the lncRNA H19, used as negative control, resulted unaffected by YK-4-279 treatment both in the RT-qPCR and in the array screening (Supplementary Fig. S1E).
MIR17-HG, AFAP1-AS1, and HULC showed the strongest deregulation in our analysis. To investigate the role of these lncRNAs in ES, we first mimicked the effect of YK-4-279 by overexpressing (AFAP1-AS1) or knocking down (HULC and MIR17-HG) their expression. Downregulation of MIR17-HG and up-regulation of AFAP1-AS1 did not affect ES cell
clonogenicity (Fig.2A, B). On the contrary, silencing of HULC strongly impaired the capability of ES cells to proliferate and form clones (Fig.2C, D), without inducing cell death (Fig.2E). Interestingly, HULC RNA levels were higher in the aggressive and metastatic TC-71 and SK- N-MC cell lines, in comparison with LAP-35 and RD-ES, originated from primary tumors (Fig.2F).
Taken together, these results point to a potential role of the HULC lncRNA in ES tumorigenicity and suggest that its downregulation might be involved in the antitumorigenic activity of the small molecule YK-4-279.
⦁ HULC sponges miR-186 in Ewing sarcoma cells
LncRNAs impact gene expression programs by taking part to different mechanisms of regulation, such as transcription, mRNA processing and translation [16]. HULC is aberrantly up‐regulated in a wide spectrum of human cancers [26-29] and contributes to tumor progression by regulating multiple pathways [30]. To accomplish this function, HULC can act as an endogenous sponge by down‐regulating microRNA activities, including those of miR‐ 372 [31], miR‐107 [32] and miR-186 [33]. To ascertain the role of HULC in the pathogenesis of ES, we first evaluated its localization in TC-71 ES cells. Cell fractionation experiments showed a predominantly cytoplasmic localization of HULC in ES cells (Fig.2G), suggesting a putative sponge activity. In-silico analysis supported this hypothesis and revealed several miRNA binding sites in the nucleotide sequence of HULC lncRNA. In particular, 13 putative miRNA binding sites were identified (Fig.3A) (see methods for details), with miR-218-5p and miR-186-5p showing the highest binding scores (Supplementary Fig.S2A).
To investigate the ability of HULC as a sponge toward miR-186 and miR-218, we first evaluated their binding by generating a reporter construct with the HULC sequence harboring
miR-186 and miR-218 binding sites downstream of the Renilla luciferase in psicheck II vector. This construct was co-transfected in TC-71 cells with either miR-186 or miR-218 oligonucleotide mimics, or scrambled mimic miRNA (scr miR). A significant reduction of the Renilla/Ffly ratio was observed in miR-186 and miR-218 transfected cells, confirming the ability of these miRNAs to bind HULC sequence (Fig.3B). Notably, the unrelated miR-663a mimic was not able to affect Renilla/Ffly activity (Fig.3B).
Next, we generated reporter constructs sensitive to miR-186 (psi-miR-186 sensor) or to miR-218 (psi-miR-218 sensor), by cloning a double copy of the reverse complement of the pre-miR sequences downstream of the Renilla reporter (Supplementary Fig.S2B). Interestingly, co-transfection of the miR-sensors with the mimics (miR-186 or miR-218) exhibited a significant repression of Ren/Ffly activity compared to control cells, confirming the efficiency of the reporter system in the detection of variation of miR-186 and miR-218 levels (Supplementary Fig.S2B). A competition assay performed by using the psi sensor plasmids and increasing amount of HULC indicated that the repression of Ren/Ffly activity induced by miR-186 was efficiently rescued by the transfection of HULC RNA. These results confirm the ability of HULC in subtracting miR-186 from the binding to target sequences, such as the psi- miR-186 sensor (Fig.3C and Supplementary Fig.S2C). Moreover, the overexpression of miR- 186 in TC-71 cells was able to reduce the endogenous levels of HULC as well as those ectopically induced by pcHULC transfection (Supplementary Fig.S2D). In the case of miR- 218, only a slight increase in the Ren/Ffly activity was detected, indicating a milder sponge activity exerted by HULC (Fig.3D).
⦁ miR-186 expression improves prognosis of Ewing sarcoma patients
Next, to evaluate the relevance of miR-186 to Ewing sarcoma prognosis, we scanned array dataset of ES patients by using R2 online tool (https://hgserver1.amc.nl/cgi- bin/r2/main.cg) Interestingly, Kaplan Meier analyses of event-free and overall survival from 64 ES patients [25] showed a positive correlation between miR-186 expression and patient prognosis (Fig.3E), suggesting a tumor suppressive function. In line with this, the overexpression of a specific miR-186 mimic was able to reduce the clonogenicity of TC-71 cells (Fig.3F). Furthermore, co-treatment of ES cells with YK-4-279 and mimic miR-186 enhanced the effect of the drug by sensitizing ES cells to the treatment (Fig.3G and H).
Collectively, these experiments highlight a role for miR-186 as a potential tumor suppressor in ES pathogenesis.
⦁ The HULC- miR-186 axis affects Twist1 expression in Ewing sarcoma cells.
Given these findings, the physiological role of HULC in ES could be exerted trough the modulation of miR-186 target genes. Among them, the most known in tumor biology is the evolutionary conserved basic helix-loop-helix (bHLH) transcription factor TWIST1 which has been recently associated to the progression of ES [34]. To this regard, low levels of TWIST1 correlate to a significant increase of event-free and overall survival of ES patients (Fig.4A), indicating a potential role of this oncogene in ES pathogenesis. Accordingly, TWIST1 protein expression is higher in the metastatic TC-71 and SK-N-MC with respect to LAP-35 and RD- ES cells (Fig.4B).
To verify the putative regulation of miR-186 on TWIST1, ES cells were transfected with either miR-186 or a scrambled miR-mimic. As shown in Fig.4C, the miR-186 overexpression strongly reduced TWIST1 protein content without affecting its mRNA levels, both in the TC-71 and in the SK-N-MC ES cells. The negative feedback of HULC toward miR-186 prompted us
to investigate whether changes in HULC expression could impact TWIST1 protein level. Thus, TC-71 and SK-N-MC cells were transfected with HULC antisense oligonucleotides (ASO), and a significant reduction of TWIST1 protein was observed (Fig.4D), suggesting a functional role of HULC lncRNA in the regulation of miR-186 target genes. In particular, the ASO- dependent lowering of HULC would allow the release of miR-186, thus enabling its binding to TWIST1 3’UTR and inducing the repression of its translation. In line with this, a slight but significant increase of TWIST1 protein was observed in HULC-overexpressing TC-71 cells transfected with pcHULC (Fig.4E). Similarly, TC-71 and SK-N-MC cells treated with YK-4-279 displayed a substantial decrease of TWIST1 protein, without changes in the mRNA levels (Fig.4F), suggesting that HULC downregulation induced by YK-4-279 (Fig.1 and Supplementary Fig. S2E) could cause the release of miR-186, thus driving the repression of TWIST1 oncogene in a post-transcriptional manner. Interestingly, the expression of HMGA2, a well-known target of the HULC-miR-186 axis was unaffected (Supplementary Fig.S2F). Taken together, these results identify a novel molecular axis formed by HULC-miR-186- TWIST1 in ES, playing a direct role in the YK-4-279 antitumorigenic activity.
To functionally test this hypothesis, we ectopically modulated TWIST1 levels in TC-71 cells and evaluated their clonogenicity upon YK-4-279 treatment. Remarkably, overexpression of TWIST1 oncogene (Fig.5A) enhanced the clonogenic potential of TC-71 cells in the presence of YK-4-279 (Fig.5B). Particularly, clones generated by TWIST1- overexpressing cells were still detectable at the highest YK-4-279 treatment (1M) and their number even overlapped those of the control pcDNA3.1 cultured with a YK-4-279 lower concentration (0.75M). Conversely, downregulation of TWIST1 expression by siRNA (Fig. 5C), increased ES cells sensitivity toward YK-4-279 treatment (Fig.5D).
Collectively, these experiments highlight a key role of TWIST1 in the resistance of ES cells toward the YK-4-279 chemical compound and disclose the HULC-miR-186-TWIST1 axis as a new player in ES chemoresistance with enormous therapeutic potential.
To further confirm our hypothesis, we measured the expression levels of HULC and TWIST1 in a pool of TC-71 cells which exhibit an increased resistance to YK-4-279 as shown by the significant rise of their IC50 value and clonogenicity (Fig.5E and F). Importantly, YK-4- 279-resistant cells express high levels of both HULC lncRNA and TWIST1 protein, supporting the role of these two molecules in the YK-4-279-mediated chemoresistance (Fig.5G). According to their oncogenic role, most of the YK-4-279-sensitive lncRNAs identified by our initial array, were found to be upregulated in YK-resistant cells (Supplementary Fig. S3). In addition, the sensitivity of these cells to anti-cancer drugs currently used in ES therapy, such as vincristine and doxorubicin, was similar to that observed for parental control cells (Fig.5H). Remarkably, YK-resistant cells showed higher sensitivity to etoposide treatment, suggesting potential combination therapies to counteract YK-4-279 resistance (Fig. 5H).
⦁ Discussion
The development of resistance to chemotherapy remains the main reason for treatment failure in patients with ES [35]. Thus, alternative strategies are urgently needed. YK-4-279 drug is a promising compound recently developed to inhibit EWS-FLI1/DHX9 interaction and to counteract the oncogenic program orchestrated by EWS-FLI1 [10, 12]. A YK-4-279 derivative is now being evaluated in phase 1 clinical trials for Ewing sarcoma patients (NCT02657005). Nevertheless, the molecular mechanism operated by YK-4-279 to achieve its antitumorigenic effect in ES is still far to be completely elucidated. In particular, the contribution of noncoding RNAs (ncRNAs) has never been addressed yet. Given the role of
EWS-FLI1 in regulating the expression of different ncRNAs species [36-38] and the direct involvement of DHX9 in miRNA biogenesis [39], the impact of the inhibition of EWS- FLI1/DHX9 interaction by YK-4-279 on ncRNA molecules could be crucial to address ES pathogenesis.
In this study, we find that HULC, a lncRNA highly expressed in hepatocellular carcinoma [26], is downregulated in ES cells upon YK-4-279 treatment. HULC lncRNA binds to miR-186 and inhibits its function, by acting as a molecular sponge. miR-186-binding site on HULC appears very strong, as predicted by in silico analysis performed on HULC sequence. Moreover, gene reporter assays (Fig.3) confirmed the interaction between miR-186 and HULC in ES cells. In line with our findings, Wang and colleagues reported that in liver cancer HULC mediates the up-regulation of HMGA2 by acting post-transcriptionally as a ceRNA toward miR-186. Mechanistically, miR-186 was capable to decrease the expression of HMGA2 via targeting the 3′-UTR of HMGA2 mRNA. By contrast, HULC sponge activity toward miR-186 led to the increase of HMGA2 expression promoting liver cancer growth [33]. In ES cells, the expression of HMGA2 was not affected by YK-4-279 treatment, and HMGA2 expression did not correlate with worse prognosis in ES patients (Supplementary Fig.S2F-G).
In our context, the physiological significance of the interaction between HULC and miR- 186 is emphasized by the fact that YK-4-279-induced downregulation of HULC reduces ES cell proliferation and clonogenicity through the downregulation of the oncogenic protein TWIST1, a phenotype that is recapitulated by miR-186 overexpression. Indeed, the increase
of miR-186, as well as the decrease of HULC, leads to a similar reduction of TWIST1 protein without affecting its mRNA levels. Thus, HULC and miR-186 work together at the post- transcriptional regulation of TWIST1 mRNA, with HULC embedded upstream of miR-186. This finding highlights the functional relevance of HULC in ES tumorigenesis and provides
new insights into the molecular axis miR-186-TWIST1, previously documented in other cancer types [40, 41]. Remarkably, Kaplan Meier analyses strongly support the relevance of both these molecules in ES pathogenesis. High levels of miR-186, as well as low levels of TWIST1, correlate to a better prognosis of ES patients, suggesting that the identification of molecular mechanisms involved in the regulation of their expression could be relevant to implement current therapeutic strategies.
TWIST1 is a transcription factor belonging to the basic helix-loop-helix twist family bHLH [42]: it is a regulator of mesenchymal phenotypes and has been proposed as an oncogenic transcription factor involved in tumor metastasis [42]. Overexpression of TWIST1 in non-metastatic ES cell lines, which physiologically express low levels of TWIST1, leads to the increase of their migration ability [34]. Notably, TWIST1 is upregulated in cisplatin- resistant cells and is involved in the acquired resistance to taxol in ovarian and prostate cancer cells [43, 44]. Moreover, in skin tumors it has been shown that TWIST1 is expressed at the earliest step of tumorigenesis, suggesting a role in tumor initiation and progression, independently by its ability to induce metastasis [45]. In particular, the downregulation of TWIST1 reduces cell proliferation in a p53 independent manner [45]. In line with this, it has been reported that Twist1 knock-down inhibits the growth of synovial sarcoma xenografts and Twist1-depleted cells show G1 cell cycle arrest and reduced sphere-forming properties [46]. Hence, the oncogenic role of TWIST1 can be executed through alternative mechanisms acting in parallel with the metastatic process and/or during different stages of tumor development. In our context, the ectopic modulation of TWIST1 expression affects TC-71 clonogenicity (Fig.5), strongly supporting the hypothesis that TWIST1 functions as a regulator of tumor growth in ES.
Our results document a novel molecular axis triggered by YK-4-279 where the expression of TWIST1 oncogene is modulated through the sponge activity of HULC lncRNA toward miR-186 (Fig. 6). As recently documented by Xiong and colleagues in hepatocellular carcinoma, the sponge activity of HULC is associated to the acquisition of chemoresistance [47]. Interestingly, we found that HULC is downregulated upon YK-4-279 treatment and this lowering is a critical step to sensitize ES cells to the treatment. Remarkably, HULC and TWIST1 levels are strongly up-regulated in YK-4-279-resistant TC-71 cells, supporting the hypothesis that the modulation of HULC expression, as well as that of its molecular targets, contribute to the acquisition of chemoresistance of ES cells. To this regard, YK-resistant cells showed higher sensitivity to etoposide treatment, suggesting potential combination therapies to counteract YK-4-279 resistance. These findings offer the possibility of a novel and alternative targeted-strategy to address ES pathology.
Collectively, our results disclose a novel oncogenic regulatory circuit related to the sponge activity of HULC lncRNA toward miR-186 in ES and identify potential biomarkers that could be useful during the clinical development of YK-4-279 derivatives.
Acknowledgements
The authors wish to thank Drs. Roberta Maestro and Sara Piccinin for the generous gift of the pcTWIST1 plasmid, Dr. Elisabetta Volpe for technical assistance in the flow cytometric analysis.
This work was supported by grants from the Associazione Italiana Ricerca sul Cancro (AIRC; IG21877) and Sarcoma Foundation of America (SFA 582104) to M.P.P.
N.M. and R.P. were supported by scholarships from Fondazione Umberto Veronesi.
Author contributions
N.M. and M.P.P. designed the research and wrote the manuscript. N.M. performed most of the experiments. D.F. contributed to the RT-qPCR analyses. R.P. performed the cellular fractionation and helped in the R2 analysis. M.P.P. supervised the project.
Competing Interests
The authors declare no conflict of interest.
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Figure Legends
Figure 1. An RT-qPCR- based array of 84 cancer-related lncRNAs identifies HULC as a prominent lncRNA downregulated by YK-4-279 treatment in TC-71 cells. Total RNAs extracted from TC-71 cells cultivated in presence or not of YK-4-279 for 24 hours were reverse transcribed and submitted to qPCR analysis by using RT2 lncRNA PCR array Human Cancer Pathway Finder (Qiagen). Ct values (cut off: 35) obtained by each sample (triplicate) were assigned to DMSO (ctrl) and YK-4-279 (YK) treatment groups and normalized by using an arithmetic mean of 4 different housekeeping gene. Results are shown as fold change. A) Hierarchical clustering of the entire dataset shown as a heat map with dendrograms indicating co-regulated genes across groups or individual samples. B) Volcano plot showing the significant gene expression changes by combining a p-value statistical test with the fold regulation change (threshold set=1.5). Downregulated lncRNAs are represented as blue circles, whereas the upregulated are red. C) Histogram showing the fold change of the identified lncRNAs. D) RT-qPCR analysis showing the expression levels (normalized to GAPDH) of AFAP1-AS1, HULC, CCAT2, SUMO1P3, HIF1A-AS2, MIR17-HG, NRON,
MALAT1, NEAT1, CCAT1 in DMSO ctrl and YK-4-279 samples. Statistical analysis was performed by unpaired Student’s t-test (*p<0.05; **p<0.01; ***p<0.001).
Figure 2. HULC depletion impairs TC-71 cellular growth. Colony assay performed on TC-71 cells transfected with either scrambled or specific antisense oligonucleotide (ASO) targeting MIR17-HG (MIR17-HG ASO) (A) and HULC (HULC ASO) (C) or with pcAFAP1-AS1 (B).
Histograms on the left show the expression level these lncRNAs 24 hours after transfection revealed by RT-qPCR analysis. Histograms on the right represent the number of colonies (n=3, mean±SD). D) Line graph showing the number of TC-71 cells measured at 24, 48 and
72 hours after transfection with ASO HULC or scramble ASO (scr ASO). Cell images were taken at 48 hours by using ZOE Fluorescent Cell Imager (BIO-RAD). E) Bar graphs show the
% of viable versus dead cells monitored by Trypan blue. F) HULC expression analyzed by RT-qPCR in TC-71, SK-N-MC, LAP-35 and RD-ES cell lines (* refers to SK-N-MC vs LAP-35 and RD-ES; # refers to TC-71 vs LAP-35 and RD-ES). Statistical analysis was performed by unpaired Student’s t-test (#, *p<0.05; ##, **p<0.01; ***p<0.001). G) Cytosolic and nuclear fractions of TC-71 cells were analyzed to monitor the localization of HULC lncRNA. RNA was isolated from each fraction. Histograms show the percentage of U1snRNP (on the top), HULC (in the middle), and DHX9 mRNA (on the bottom) co-sedimenting in each fraction (mean ± S.D.).
Figure 3. HULC sponges miR-186. A) Bioinformatic search (www.microRNA.org) for putative miRNA binding sites in the nucleotide sequence of HULC RNA. B) Schematic representation of miR-186 and miR-218 pairing sites in HULC RNA sequence. On the bottom, histograms show the Ren/Ffly ratio in lysates obtained from TC-71 cells transfected with psiHULC together with scr miR, miR-186, miR-218 or miR-663a miRNA mimics. The experiment was performed at least three times and each biological sample was measured in triplicate. C, D) Competition assay between psi-miR-186 sensor and pcHULC for miR-186 (C) and between psi-miR-218 sensor and pcHULC for miR-218 (D). Data are shown as mean of Ren/Ffly ratio
± SD of at least three independent experiments. The black bars indicate the Ren/Ffly ratio obtained in TC-71 cells transfected with the sensor plasmid, pcDNA3.1 and the relative mimic miRNA. Values measured after transfection of increasing amount of pcHULC (from 0.5 to 0.8
g; black triangle) are represented by white bars. Statistical analysis was performed by unpaired Student’s t-test (*p<0.05; **p<0.01; ***p<0.001). E) Kaplan Meier plot generated by
R2 webcite showing the correlation between miR-186 expression and the overall (left) or event-free (right) survival of 64 ES patients (dataset: Mixed Ewing Savola; subset: diagnosis; subset track: ewing). F) Colony assay performed on TC-71 cells transfected with either miR- 186 or scramble (scr miR) miRNA mimics. On the right, histograms show the number of colonies in each sample. G) The clonogenicity of these cells has been also monitored in presence of effective doses of YK-4-279 (0.75-1M) and results are plotted as mean ± SD of at least three independent experiments (H). Statistical analysis was performed by unpaired Student’s t-test (*p<0.05; **p<0.01; ***p<0.001).
Figure 4. The HULC-miR-186 axis affects the expression of TWIST1 protein in ES cell lines.
A) Kaplan Meier plot generated by R2 tool showing the correlation between TWIST1 expression and the overall (left) or event-free (right) survival of 64 ES patients (dataset: Mixed Ewing Savola; subset: diagnosis; subset track: ewing). B) Western Blot analysis showing the protein content of TWIST1 in a cohort of ES cell lines originated from metastatic (M) or primary (P) tumors. GAPDH expression was used as housekeeping loading control. C) Expression analysis of TWIST1 in TC-71 and SK-N-MC cells transfected either with miR-186 or scrambled miR- mimics. A representative image of the western blot and the relative quantification of TWIST1 protein levels from three independent experiments are shown (mean
± SD). The histogram below refers to the RT-qPCR analysis carried out to monitor the relative expression of TWIST1 mRNA normalized to GAPDH. D) Expression analysis of TWIST1 in TC-71 and SK-N-MC cells transfected either with HULC ASO or scrambled ASO. A representative image of the western blot and the relative quantification of TWIST1 protein levels from three independent experiments are shown (mean ± SD). The histogram below refers to the RT-qPCR analysis carried out to monitor the relative expression of TWIST1
mRNA normalized to GAPDH. E) Western blot analysis showing the protein levels of TWIST1 in TC-71 cells transfected with pcHULC or pcDNA3.1 and relative quantification measured as mean of TWIST1 normalized to GAPDH expression from three independent experiments. F) Expression analysis of TWIST1 levels in TC-71 and SK-N-MC cells exposed to YK-4-279 (2M) at protein (western blot) and mRNA levels (RT-qPCR). Statistical analysis was performed by unpaired Student’s t-test (*p<0.05; **p<0.01; ***p<0.001).
Figure 5. The expression of TWIST1 affects ES sensitivity to YK-4-279 treatments. A) Western blot analysis showing the TWIST1 levels in TC-71 transfected with either pcTWIST1 or pcDNA3.1 plasmids. The bar graph refers to the quantification of TWIST1 on GAPDH levels from three independent experiments (mean ± SD). B) Colony assay performed on TC- 71 cells transfected by pcTWIST1 or pcDNA3.1 with relative quantification histogram showing the number of colonies in presence or not of effective doses of YK-4-279. C) Western blot analysis carried out to monitor the protein levels of TWIST1 in TC-71 cells transfected with Twist1 siRNA or scr siRNA. Histogram show the relative levels of TWIST1 normalized to GAPDH as mean of at least three experiments. D) Colony assay performed on TC-71 cells transfected by TWIST1 siRNA or scrambled siRNA with relative quantification histogram showing the number of colonies in presence or not of effective doses of YK-4-279. E) Dose- response curve of YK-4-279-TC-71 resistant (YK Res), even grown for 1 week in a YK-4-279- free media (YK Res 1WF) vs DMSO control cells (ctrl). (IC50; YK Res: 1.77 µM, YK Res 1WF:
1.9 µM, ctrl: 1.5 µM). On the right, a representative image of ctrl and YK Res cells exposed to 1.5µM of YK-4-279 is shown. F) Colony assay of YK Res and ctrl TC-71 cells with relative quantification histogram showing the number of colonies in presence or not of effective doses of YK-4-279. G) Western blot and RT-qPCR analysis showing the differential expression of
TWIST1, EWS-FLI1 and HULC in YK-4-279-TC-71 resistant (YK Res) vs DMSO control cells (ctrl). Data are represented as mean ± SD of three independent experiments. Statistical analysis was performed by unpaired Student’s t-test (*p<0.05; **p<0.01; ***p<0.001). H) Viability (measured by MTS assay) of YK Res and ctrl cells treated with different concentrations of Vincristine (Vcr), Etoposide (Eto) and Doxorubicin (Dox) for 48 hours. Each treatment was able to significantly reduce cell viability (p<0.001, Two-way analysis of variance, Bonferroni correction). The unpaired Student’s t-test is referred to YK Res and ctrl groups after Etopoiside treatment (***p<0.001).
Figure 6. Schematic representation of HULC sponge activity toward miR-186 in ES and its role in the YK-4-279-mediated effect through TWIST1 regulation.
A B
C D
E F
A B
C D
G H
EWS-FLI1 TWIST1 GAPDH
Highlights
⦁ HULC-miR-186-TWIST1 axis is a new player in Ewing sarcoma transformation;
⦁ HULC promotes the expression of TWIST1 oncogene by sponging miR- 186
⦁ Regulation of TWIST1 expression is a key event in Ewing sarcoma chemoresistance.
⦁ High levels of miR-186 and low levels of TWIST1 correlate with better prognosis in ES patients.
Competing Interests
The authors declare no conflict of interest.